Assessing water permeability of aquaporins in a proteoliposome-based stopped-flow setup

Assessing water permeability of aquaporins in a proteoliposome-based stopped-flow setup

Blog Article

Summary: Aquaporins (AQPs) are water channels embedded in the cell membrane Belt that are critical in maintaining water homeostasis.We describe a protocol for determining the water permeation capacity of AQPs reconstituted into proteoliposomes.Using a stopped-flow setup, AQP embedded in proteoliposomes are exposed to an osmogenic gradient that triggers water flux.

The consequent effects on proteoliposome size can be tracked using the fluorescence of an internalized fluorophore.This enables controlled characterization of water flux by AQPs.For complete details on the Framed Picture use and execution of this protocol, please refer to Kitchen et al.

(2020).